Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA

  • Débora Oro Ferrari
  • Rafael Guerra Lund
  • Luana Zanella
  • Walter Antônio Roman Júnior
  • Sinval Adalberto Rodrigues Junior


Denture use may aggravate the occurrence of oral infections, considering it enhances microbial adherence. Aim: This study assessed the reduction of microbial loads of Candida albicans, Staphylococcus aureus, and Klebsiella oxytoca by disinfecting the polymethylmethacrylate (PMMA) of complete dentures with hydroalcoholic extract of Salvia officinalis. Additionally, the effect of such extract on the properties of PMMA was examined. Methods: Microorganisms were isolated from saliva samples collected from complete denture wearers. The hydroalcoholic extract of S. officinalis was produced according to the Brazilian Pharmacopoeia 5. The PMMA specimens (n=188) were immersed in microbial inoculum and incubated at 37°C for 16 hours per day. Then, they were subjected to a disinfection protocol for 30 days. The specimens were divided into five treatment groups: sterile saline solution (0.85%; control), 0.2% chlorhexidine digluconate, and hydroalcoholic extract of S. officinalis (0.2%, 0.8%, and 1.16%). Microorganism adherence to the PMMA surface was also assessed, as well as surface roughness (Ra in μm) and color stability of the PMMA (mean ΔE). Changes in microbial load and surface roughness after the disinfection protocol were verified with paired t-test. Substances at day 10, adherence, and color stability were compared by the Kruskall-Wallis and Mann-Whitney tests, and one-way ANOVA was used to compare substances at the beginning and end of the experiment (α=0.05). Results: The 1.16% S. officinalis extract significantly reduced the microbial load of all the microorganisms after 30 days of disinfection (p<0.05). The microbial load of K. oxytoca was also reduced at lower concentrations of the S. officinalis extract (0.2% and 0.8%) (p<0.02). Antimicrobial and anti-adherent effects against microorganisms isolated from the oral cavity were observed. There was no significant change in surface roughness (p>0.05) and color stability was significantly higher in the control group (p<0.0001). Conclusions: The hydroalcoholic extract of S. officinalis may be used as a disinfectant solution for dentures.


1. Santos CM, Hilgert JB, Padilha DMP, Hugo FN. Denture stomatitis and its risk
indicators in south Brazilian older adults. Gerodontology. 2010 Jun;27(2):134-40.
doi: 10.1111/j.1741-2358.2009.00295.x.
2. Martins N, Barros L, Santos-Buelga C, Henriques M, Silva S, Ferreira IC. Evaluation of bioactive
properties and phenolic compounds in different extracts prepared from Salvia officinalis L. Food
Chem. 2015 Mar 1;170:378-85. doi: 10.1016/j.foodchem.2014.08.096.
3. Vieira DRP, Amaral FMM, Maciel MCG, Nascimento FRF, Libério AS. [Plants and chemical
constituents used in dentistry: review of ethnopharmacological and antimicrobial
activity studies in oral pathogens]. Rev Bras Plantas Med. 2014;16(1):135-67.
doi: 10.1590/S1516-05722014000100020. Portuguese.
4. Zilberstein B, Quintanilha AG, Santos MA, Pajecki D, Moura EG, Alves PR et al. Digestive tract
microbiota in healthy volunteers. Clinics (Sao Paulo). 2007 Feb;62(1):47-54.
5. Valentini F, Luz MS, Boscato N, Pereira-Cenci T. Biofilm formation on denture liners in a randomised
controlled in situ trial. J Dent. 2013 May;41(5):420-7. doi: 10.1016/j.jdent.2013.02.012.
6. Mese A, Mese S. Effect of microwave energy on fungal growth of resilient denture liner material.
Biotechnol Biotechnol Equip. 2014 Apr;21(1):91-3. doi: 10.1080/13102818.2007.10817422.
7. Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on
supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol.
1995 Jan;22(1):1-14.
8. Vasconcelos LR, Consani RLX, Mesquita MF, Sinhoreti MA. Effect of chemical and microwave
disinfection on the surface microhardness of acrylic resin denture teeth. J Prosthodont. 2013
Jun;22(4):298-303. doi: 10.1111/jopr.12009.
9. Muscat Y, Farrugia C, Camilleri L, Arias-Moliz MT, Valdramidis V, Camilleri J. Investigation of
acrylic resin disinfection using chemicals and ultrasound. J Prosthodont. 2018 Jun;27(5):461-468.
doi: 10.1111/jopr.12511.
10. Panariello BH, Izumida FE, Moffa EB, Pavarina AC, Jorge JH, Giampaolo ET. Effect of mechanical
toothbrushing combined with different denture cleansers in reducing the viability of a multispecies
biofilm on acrylic resins. Am J Dent. 2016 Jun;29(3):154-60.
11. Peracini A, Machado Andrade I, Oliveira VC, Macedo AP, Silva-Lovato CH, Oliveira Pagnano V, et al.
Antimicrobial action and long-term effect of overnight denture cleansers. Am J Dent. 2017 Apr;30(2):101-8.
12. Guiotti AM, Cunha BG, Paulini MB, Goiato MC, Dos Santos DM, Duque C et al. Antimicrobial activity of
conventional and plant-extract disinfectant solutions on microbial biofilms on a maxillofacial polymer
surface. J Prosthet Dent. 2016 Jul;116(1):136-43. doi: 10.1016/j.prosdent.2015.12.014.
13. Kon KV, Rai MK. Plant essential oils and their constituents in coping with multidrug-resistant bacteria.
Expert Rev Anti Infect Ther. 2012 Jul;10(7):775-90. doi: 10.1586/eri.12.57.
14. Lorenzi H, Matos FJA. [Medicinal plants in Brazil: native and exotic]. São Paulo: Instituto Plantarum;
2002. 512 p. Portuguese.
15. ESCOP 1996. Salviae folium (Sage leaf). Monographs on the Medicinal Use of Plant Drugs. Exeter.
UK: European Scientific Cooperative on Phytotherapy; 1997.
16. Wichtl M. Herbal Drugs and Phytopharmaceuticals. Boca Raton, FL: CRC Press; 1994. p. 440-3.
17. Guiotti AM, Goiato MC, Dos Santos DM, Vechiato-Filho AJ, Cunha BG, Paulini MB et al. Comparison
of conventional and plant-extract disinfectant solutions on the hardness and color stability
of a maxillofacial elastomer after artificial aging. J Prosthet Dent. 2016 Apr;115(4):501-8.
doi: 10.1016/j.prosdent.2015.09.009.
18. Roman Junior WA, Picolli AL, Morais B, Loeblein M, Schönell AP. [Antiulcerogenic activity of
aqueous extract of Salvia officinalis L. (Lamiaceae)]. Rev Bras Pl Med. 2015;17(Suppl 1):774-81.
doi: 10.1590/1983-084X/14_059. Portuguese.
19. Oliveira KB, Oliveira BH. HPLC/DAD determination of rosmarinic acid in Salvia officinalis:
sample preparation optimization by factorial design. J Braz Chem Soc. 2013;24(1):85-91.
doi: 10.1590/S0103-50532013000100012.
20. National Health Surveillance Agency (Brazil). [Brazilian pharmacopoeia]. 5. ed. Brasília: Anvisa; 2010.
546 p. Portuguese.
21. Abu-Bakr N, Han L, Okamoto A, Iwaku M. Color stability of compomer after immersion in various
media. J Esthet Dent. 2000;12(5):258-63.
22. Commission Internationale de l’Eclairage - CIE. Colorimetrye official recommendations of the
Commission Internationale de l’Eclairage (CIE Publication n. 15 – E-1.3.1). Vienna (France): Bureau
Central de la CIE; 1996.
23. Tondo EC, Machado TRM, Malheiros Pda S, Padrão DK, de Carvalho AL, Brandelli A. Adhesion and
biocides inactivation of Salmonella on stainless steel and polyethylene. Braz J Microbiol. 2010
Oct;41(4):1027-37. doi: 10.1590/S1517-838220100004000022.
24. Sookto T, Srithavaj T, Thaweboon S, Thaweboon B, Shrestha B. In vitro effects of Salvia
officinalis L. essential oil on Candida albicans. Asian Pac J Trop Biomed. 2013 May;3(5):376-80.
doi: 10.1016/S2221-1691(13)60080-5.
25. Fournomiti M, Kimbaris A, Mantzourani I, Plessas S, Theodoridou I, Papaemmanouil V, et al.
Antimicrobial activity of essential oils of cultivated oregano (Origanum vulgare), sage (Salvia
officinalis), and thyme (Thymus vulgaris) against clinical isolates of Escherichia coli, Klebsiella
oxytoca, and Klebsiella pneumoniae. Microb Ecol Health Dis. 2015 Apr 15;26:23289.
doi: 10.3402/mehd.v26.23289.
26. Koslowska M, Laudy AE, Przybyl J, Ziarno M, Majewska E. Chemical composition and
antibacterial activity of some medicinal plants from Lamiaceae family. Acta Pol Pharm.
2015 Jul-Aug;72(4):757-67.
27. Horiuchi K, Shiota S, Kuroda T, Hatano T, Yoshida T, Tsuchiya T. Potentiation of Antimicrobial Activity
of Aminoglycosides by Carnosol from Salvia officinalis. Biol Pharm Bull. 2007 Feb;30(2):287-90.
28. Kiesow SA, Sarembe S, Pizzey RL, Axe AS, Bradshaw DJ. Material compatibility and antimicrobial
activity of consumer products commonly used to clean dentures. J Prosthet Dent. 2016
Feb;115(2):189-198.e8. doi: 10.1016/j.prosdent.2015.08.010.
29. Gonçalves LFF, Silva Neto DR, Bonan RF, Carlo HL, Batista AUD. [Complete and Partial
Removable Dentures Cleansing Methods. Rev Bras Cienc Saude. 2011;15(1):87-94.
doi: 10.4034/RBCS.2011.15.01.13. Portuguese.
30. Foster TJ, Geoghegan JA, Ganesh VK, Höök M. Adhesion, invasion and evasion: the many functions
of the surface proteins of Staphylococcus aureus. Nat Rev Microbiol. 2014 Jan;12(1):49-62.
doi: 10.1038/nrmicro3161.
31. Moffa EB, Giampaolo ET, Izumida FE, Paravina AC, Machado AL, Vergani CE. Color stability of relined
dentures after chemical disinfection. A randomised clinical trial. J Dent. 2011 Dec;39 Suppl 3:e65-71.
doi: 10.1016/j.jdent.2011.10.008.
32. Schwindling FS, Rammelsberg P, Stober T. Effect of chemical disinfection on the surface
roughness of hard denture base materials: a systematic literature review. Int J Prosthodont.
2014 May-Jun;27(3):215-25. doi: 10.11607/ijp.3759.
How to Cite
FERRARI, Débora Oro et al. Denture disinfection using Salvia officinalis L.: microbial load and selected properties of PMMA. Brazilian Journal of Oral Sciences, [S.l.], p. e18901, jan. 2019. ISSN 1677-3225. Available at: <>. Date accessed: 17 july 2019. doi:
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