Effects of primer excess on marginal adaptation, nanoleakage and bond strength of adhesive systems after aging

  • Suelem Chasse Barreto
  • Isabel Ferreira Barbosa
  • Gisele Damiana da Silveira Pereira
  • Carlos Tadeu dos Santos Dias
  • Luís Alexandre Maffei Sartini Paulillo


There is no consensus about the amount of primer inserted into the cavity. Aim: This study aimed to evaluate the effects of the amount of primer used on dentin microtensile bond strength (μTBs), nanoleakage and marginal sealing, following thermomechanical aging. Methods: 48 human third molars were selected and a box-shaped class I cavity was constructed to maintain enamel margins. Teeth were randomly assigned to 6 experimental groups (n=8). For the bonding protocols of the restorative procedure, two adhesive systems were used: three-step etch-and-rinse and two-step self-etch. Epoxy resin replicas of the occlusal surface were made, and the specimens were submitted to thermomechanical aging. Newer replicas were obtained after thermomechanical aging, and marginal adaptation was observed using SEM. To obtain sections (0.7 x 0.7 mm) for testing at a tension of 0.5 mm/min, teeth were serially sectioned in the buccallingual direction, parallel to the occlusal surface. Failure mode was then obtained. For nanoleakage evaluation, one section of each tooth was immersed in AgNO3 and evaluated using SEM. The μTBs data were submitted to a two-way ANOVA and Tukey’s test (α= 0.05) Results: One drop of primer promoted higher μTBs than two or three drops. Besides, the three-step etch-and-rinse promoted greater μTBs (19.78) than the two-step self-etch adhesive (12.23). The increase in the amount of primer was directly proportional to the increase of infiltration. All groups exhibited more gaps after thermomechanical aging. Conclusion: Using an excess of primer is not recommended because it reduces the μTBs and forms an unsatisfactory hybrid layer.


1. Bacelar-Sá R, Giannini M, Ambrosano GMB, Bedran-Russo AK. Dentin sealing and bond strength
evaluation of Hema- free and multi mode adhesive to biomodified dentin. Braz. Dent. J.
2017;28(6):731-7. doi: 1590/0103-6440201701522.
2. Marsiglio AA, Almeida JCF, Hilgert LA, DÁlpino PHP, Garcia FCP. Bonding to dentin as a function of
air-stream temperatures for solvent evaporation. Braz Oral Res. 2012 May-Jun;26;(3):282-7.
3. Rodrigues SB, Collares FM, Leitune VCB, Schneider LFJ, Ogliari FA, Petzhold CL, et al. Influence
of hydroxyethyl acrylamide addition to dental adhesive resin. Dent Mater. 2015;31:1579-86.
doi: 10.1016/j.dental.2015.10.005.
4. Araújo -Neto VG, Nobre CFA, De Paula DM, Souza LC, Silva JC, Moreira MM et al. Glyceroldimethacrylate
as alternative hydroplilic monomer for HEMA replacement in simplified adhesives.
J Mech Behav Biomed Mater. 2018;82:95-101. doi: 10.1016/j.jmbbm.2018.03.022.
5. Munchow EA, Valente LL, Bossardi M, Priebe TC, Zanchi CH, Piva E. Influence of surface moisture
condition on the bond strength to dentin of etch-and-rinse adhesive systems. Braz J Oral Sci.
2014;13(3):182-6. doi: 10.1590/1677-3225v13n3a04.
6. Pashley DH, Tay FR, Carvalho RM, Rueggeberg FA, Agee KA, Carrilho M, et al. From dry bonding to
water-wet bonding to ethanol-wet bonding. A review of the interactions between dentin matrix and
solvated resins using a macromodel of the hybrid layer. Am J Dent. 2007 Feb;20(1):7-20.
7. Cadenaro M, Breschi L, Rueggeberg FA, Suchko M, Grodin E, Agee K, et al. Effects of residual ethanol
on the rate and degree of conversion of five experimental resins. Dent Mater. 2009;25(5):621-8.
doi: 10.1016/j.dental.2008.11.005.
8. De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, et al. A critical review
of the durability of adhesion to tooth tissue: methods and results. J Dent Res. 2005;84:118-32.
doi: 10.1177/154405910508400204.
9. Deng D, Yang H, Guo J, Chen X, Zhang W, Huang C. Effects of different artificial ageing
methods on the degradation of adhesive–dentine interfaces. J Dent. 2014;42:1577-85.
doi: 10.1016/j.jdent.2014.09.010.
10. Soares GP, Ambrosano GMB, Lima DANL, Marchi GM, Correr-Sobrinho L, Lovadino JR, et
al. Effect of light polymerization time, mode, and thermal and mechanical load cycling
on microleakage in resin composite restorations. Lasers Med Sci. 2014;29:545-50.
doi: 10.1007/s10103-012-1244-7.
11. Garcia- Godoy R, Krämer, Feilzer AJ, Frankenberger R. Long-term degradation of
enamel and dentin bonds: 6-year results in vitro vs. in vivo. Dent Mater. 2010;26:1113-8.
doi: 10.1016/j.dental.2010.07.012.
12. Tay FR, Pashley DH, Suh BI, Carvalho RM, Itthagarun A. Single-step adhesives are permeable
membranes. J Dent. 2002;30:371-382. doi: 10.1016/S0300-5712(02)00064-7.
13. Matos AB, Trevelin LT, Silva BTF, Francisconi-dos- Rios LF, Siriani LK, Cardoso MV.
Bonding efficiency and durability: current possibilities. Braz Oral Res. 2017;31(suppl):e57.
doi: 10.1590/1807-3107BOR-2017.vol31.0057.
14. Hass V, Dobrovolski M, Zander Grande C, Martins GC, Gordillo LAA, Rodrigues Accorinte
Mde L, et.al. Correlation between degree of conversion, resin–dentin bond strength and
nanoleakage of simplified etch-and-rinse adhesives. Dent Mater 2013;29(9):921-28.
15. Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, Van Landuyt KL. State of the art of
self-etch adhesives. Dent Mater. 2011;27(1):17-28. doi: 10.1016/j.dental.2010.10.023.
16. Watanabe I, Nakabayashi N, Pashley DH. Bonding to ground dentin by a phenyl-P self-etching primer.
J Dent Res. 1994 Jun;73(6):1212-20.
17. Inoue S, Vargas MA, Abe Y, Yoshida Y, Lambrechts P, Vanherle G, et al. Microtensile bond strength of
eleven contemporary adhesives to dentin. J Adhes Dent. 2001 Fall;3(3):237-45.
18. Van Landuyt KL, De Munck J, Snauwaert J, Coutinho E, Poitevin A, Yoshida Y, et al.
Monomer-solvent phase separation in one-step self-etch adhesives. J Dent Res. 2005;84(2):183-8.
doi: 10.1177/154405910508400214.
19. Giannini M, Makishi P, Ayres APA, Vermelho PM, Fronza BM, Nikaido T et al. Adhesive/Dentin
Interface: Self-etch adhesive systems: A literature review. Braz Dent J. 2015;26(1):3-10.
doi: 10.1590/0103-6440201302442.
20. Ikeda T, De Munck J, Shirai K, Hikita K, Inoue S, Sano H, et al. Effect of evaporation of primer
components on ultimate tensile strengths of primer- adhesive mixture. Dent Mater. 2005;21:1051-8.
21. Yang H, Guo J, Guo J, Chen H, Somar M, Yue J, et al. Nanoleakage evaluation at adhesivedentin
interfaces by different observation methods. Dent Mater J. 2015;34(5):654-62.
How to Cite
BARRETO, Suelem Chasse et al. Effects of primer excess on marginal adaptation, nanoleakage and bond strength of adhesive systems after aging. Brazilian Journal of Oral Sciences, [S.l.], v. 18, p. e191456, may 2019. ISSN 1677-3225. Available at: <https://www.fop.unicamp.br/bjos/index.php/bjos/article/view/1544>. Date accessed: 21 july 2019. doi: https://doi.org/10.20396/bjos.v18i0.8655289.
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