Bacterial microleakage at the implant-abutment interface in Morse taper implants

  • João Paulo da Silva-Neto
  • Marina de Freitas Fratari Majadas
  • Marcel Santana Prudente
  • Thiago de Almeida Prado Naves Carneiro
  • Mario Paulo Amante Penatti
  • Flávio Domingues das Neves
Keywords: dental implants, dental abutments, microbiology


Aim: To evaluate the microleakage at the implant-abutment (I-A) interface of Morse tapered implants inoculated with different volumes of bacterial suspension. Methods: Morse tapered IA sets were selected and divided in two groups depending on the type of abutment: passing screw (PS) and solid (S), and then subdivided into four subgroups (n=6) according to the suspension volume: PS1: 0.1 μL; PS3: 0.3 μL; PS5: 0.5 μL; PS7: 0.7 μL; S1: 0.1 μL; S3: 0.3 μL; S5: 0.5 μL and S7: 0.7 μL. A control test was performed to verify the presence of external contamination during the inoculation and the implants were incubated for microbiological analysis. The microleakage was evaluated every 24 h for 7 days by the clarity of solution. After this period, the implants were disassembled for confirmation of bacterial viability. Results: All the specimens with 0.7 μL and one sample of S5 presented turbidity in the control test indicating external contamination, and were excluded from the study. After 7 days of observation, none of the specimens presented positive results for microleakage and the bacterial viability was confirmed in all specimens. The 0.1 μL and 0.3 μL volumes did not present bacterial microleakage, meaning that these volumes may be inadequate for analysis. Conclusions: None of the sets evaluated showed bacterial microleakage at the I-A interface and the volume of 0.7 μL exceeded the internal capacity of the implants.


1. Goodacre CJ, Bernal G, Rungcharassaeng K, Kan JY. Clinical complications with implants and implant prostheses. J Prosthet Dent. 2003; 90: 121-32.
2. Jansen VK, Conrads G, Richter EJ. Microbial leakage and marginal fit of the implant-abutment interface. Int J Oral Maxillofac Implants. 1997; 12: 527- 40.
3. Koutouzis T, Wallet S, Calderon N, Lundgren T. Bacterial colonization of the implant-abutment interface using an in vitro dynamic loading model. J Periodontol. 2011; 82: 613-8.
4. Aloise JP, Curcio R, Laporta MZ, Rossi L, da Silva AM, Rapoport A. Microbial leakage through the implant-abutment interface of Morse taper implants in vitro. Clin Oral Implants Res. 2010; 21: 328-35.
5. Deconto MA, Salvoni AD, Wassall T. In vitro microbiological bacterial seal analysis of the implant/abutment connection in morse taper implants: a comparative study between 2 abutments. Implant Dent. 2010; 19: 158-66.
6. Tesmer M, Wallet S, Koutouzis T, Lundgren T. Bacterial colonization of the dental implant fixture-abutment interface: an in vitro study. J Periodontol. 2009; 80: 1991-7.
7. Dibart S, Warbington M, Su MF, Skobe Z. In vitro evaluation of the implantabutment bacterial seal: the locking taper system. Int J Oral Maxillofac Implants. 2005; 20: 732-7.
8. Harder S, Dimaczek B, Acil Y, Terheyden H, Freitag-Wolf S, Kern M. Molecular leakage at implant-abutment connection—in vitro investigation of tightness of internal conical implant-abutment connections against endotoxin penetration. Clin Oral Investig. 2010; 14: 427-32.
9. do Nascimento C, Pedrazzi V, Miani PK, Moreira LD, de Albuquerque RF, Jr. Influence of repeated screw tightening on bacterial leakage along the implant-abutment interface. Clin Oral Implants Res. 2009; 20: 1394-7.
10. do Nascimento C, Miani PK, Pedrazzi V, Gonçalves RB, Ribeiro RF, Faria AC, et al. Leakage of saliva through the implant-abutment interface: in vitro evaluation of three different implant connections under unloaded and loaded conditions. Int J Oral Maxillofac Implants. 2012; 27: 551-60.
11. Coelho PG, Sudack P, Suzuki M, Kurtz KS, Romanos GE, Silva NR. In vitro evaluation of the implant abutment connection sealing capability of different implant systems. J Oral Rehabil. 2008; 35: 917-24.
12. Jaworski ME, Melo AC, Picheth CM, Sartori IA. Analysis of the bacterial seal at the implant-abutment interface in external-hexagon and Morse taperconnection implants: an in vitro study using a new methodology. Int J Oral Maxillofac Implants. 2012; 27: 1091-5.
13. Steinebrunner L, Wolfart S, Bossmann K, Kern M. In vitro evaluation of bacterial leakage along the implant-abutment interface of different implant systems. Int J Oral Maxillofac Implants. 2005; 20: 875-81.
14. Silva-Neto JP, Prudente MS, Carneiro T de A, Nobilo MA, Penatti MP, Neves FD. Micro-leakage at the implant-abutment interface with different tightening torques in vitro. J Appl Oral Sci. 2012; 20: 581-7.
15. Silva-Neto JP, Nobilo MA, Penatti MP, Simamoto PC, Jr., Neves FD. Influence of methodologic aspects on the results of implant-abutment interface microleakage tests: a critical review of in vitro studies. Int J Oral Maxillofac Implants. 2012; 27: 793-800.
16. Besimo CE, Guindy JS, Lewetag D, Meyer J. Prevention of bacterial leakage into and from prefabricated screw-retained crowns on implants in vitro. Int J Oral Maxillofac Implants. 1999; 14: 654-60.
17. do Nascimento C, Miani PK, Pedrazzi V, Muller K, de Albuquerque RF Jr. Bacterial leakage along the implant-abutment interface: culture and DNA checkerboard hybridization analyses. Clin Oral Implants Res.. 2012; 23: 1168-72.
18. Gross M, Abramovich I, Weiss EI. Microleakage at the abutment-implant interface of osseointegrated implants: a comparative study. Int J Oral Maxillofac Implants. 1999; 14: 94-100.
19. Broggini N, McManus LM, Hermann JS, Medina R, Schenk RK, Buser D, et al. Peri-implant inflammation defined by the implant-abutment interface. J Dent Res. 2006; 85: 473-8.
20. Bozkaya D, Muftu S. Mechanics of the taper integrated screwed-in (TIS) abutments used in dental implants. J Biomech. 2005; 38: 87-97.
21. Donovan R, Fetner A, Koutouzis T, Lundgren T. Crestal bone changes around implants with reduced abutment diameter placed non-submerged and at subcrestal positions: a 1-year radiographic evaluation. J Periodontol. 2010; 81: 428-34.
Original Research

Most read articles by the same author(s)