Morphological aspects of dentin-pulp complex development in the offspring of rats treated with fluoxetine during pregnancy
Aim: To evaluate the morphological aspects of coronal dentinogenesis in the first molars of 1- and 5-day-old rats whose mothers were treated with fluoxetine hydrochloride during pregnancy. Methods: Twelve pregnant Wistar rats were divided randomly into three groups: group C (control), group FL (fluoxetine administered at 10 mg/kg bodyweight), and group FX (fluoxetine administered at 20 mg/kg bodyweight). Saline (0.9%) solution or fluoxetine hydrochloride was administered subcutaneously for the first 21 days of pregnancy. Subsequently, the offspring of these animals was subdivided into subgroups according to age of tooth germ development to be studied: 1 and 5 days of life. C1 and C5 (control group 1 and 5 days of age); FL1 and FL5 (groups treated with 10 mg/kg fluoxetine at 1 and 5 days of age); FX5 and FX1 (groups treated with 20 mg/ kg fluoxetine at 1 and 5 days of age). Results: No structural changes in the dentin-pulp complex of rats whose mothers were treated with fluoxetine hydrochloride were observed at either dose. Conclusions: Fluoxetine, at the doses administered during pregnancy in this study, did not alter the morphological development of the coronal dentin-pulp complex in their offspring.
2. Patel SR, Wisner KL. Decision making for depression treatment during pregnancy and the postpartum period. Depress Anxiety. 2011; 28: 589-95.
3. Patil AS, Kuller JA, Rhee EH. Antidepressants in pregnancy: a review of commonly prescribed medications. Gynecol Surv. 2011; 66: 777-87.
4. Lanza di Scalea T, Wisner KL. Antidepressant Medication use during breastfeeding. Clin Obstet Gynecol. 2009; 52: 483-97.
5. Feijó FM, Bertoluci MC, Reis C. Serotonina e controle hipotalâmico da fome: uma revisão. Rev Assoc Med Bras. 2011; 57: 74-7.
6. Canto-de-Souza A, Souza RLN, Pelá IR, Graeff FG. Involvement of the midbrain periaqueductal gray 5-HT1A receptors in social conflict induced analgesia in mice. Eur J Pharmacol. 1998; 345: 253-6.
7. Buznikov GA, Lambert HW, Lauder JM. Serotonin and serotonin-like substances as regulators of early embryogenesis and morphogenesis. Cell Tissue Res. 2001; 305: 177-86.
8. Lauder JM, Luo X, Persico AM. Serotonergic regulation of somatosensory cortical development: lessons from genetic mouse models. Devel Neurosci. 2003; 25: 173-83.
9. Bliziotes M, Eshleman A, Burt-Pichat B, Zhang XW, Hashimoto J, Wiren K, et al. Serotonin transporter and receptor expression in osteocytic MLOY4 cells. Bone. 2006; 39: 1313-21.
10. Battaglino R, Fu J, Späte U, Ersoy U, Joe M, Sedaghat L, et al. Serotonin regulates osteoclast differentiation through its transporter. J Bone Miner Res. 2004; 19: 1420-31.
11. Moiseiwitsch JRD, Lauder JM. Simulation of murine tooth development in organotypic culture by the neurotransmitter serotonin. Arch Oral Biol. 1996; 41:161-5.
12. Moiseiwitsch JRD, Raymond JR, Tamir H, Lauder JM. Regulation by serotonin of tooth-germ morphogenesis and gene expression in mouse mandibular explant cultures. Arch Oral Biol. 1998; 43: 789-800.
13. Lesot H, Brook AH. Epithelial histogenesis during tooth development. Arch Oral Biol. 2008; 19: 1-9.
14. Bei M. Molecular Genetics of Tooth Development. Curr Opin Genet Dev. 2009; 19: 504-10.
15. Goldberg M, Kulkarni AB, Young M, Boskey A. Dentin: structure, composition and mineralization. Front Biosci. 2011; 1: 711-35.
16. Pinzon RD, Kozlov M, Burch W. Histology of rat molar pulp at different ages. J Dent Res. 1967; 46: 202-8.
17. Thesleff I, Tummers M. Tooth organogenesis and regeneration. StemBook [internet]. Cambridge (MA): Harvard Stem Cell Institute; 2009.
18. Bevelander G, Hiroshi N. The formation and mineralization of dentine. Anat Rec. 1966; 156: 303-23.
19. Sasaki T, Garant PR. Structure and organization of odontoblasts. Anat Rec. 1996; 245: 235-49.
20. Goracci G, Mori G, Baldi M. Terminal end of the human odontoblasts process: a study using SEM and confocal microscopy. Clin Oral Investig. 1999; 3: 126-32.
21. Reith EJ. Collagen formation in developing molar teeth of rats. J Ultrastruct Res. 1968; 21: 383-414.
22. Reisoli E, De Lucchini S, Nardi I, Ori M. Serotonin 2B receptor signaling is required for craniofacial morphogenesis and jaw joint formation in Xenopus. Development. 2010; 137: 2927-37.
23. Bliziotes MM, Eshleman AJ, Zhang XW, Wiren KM. Neurotransmitter action in osteoblasts: expression of a functional system for serotonin receptor activation and reuptake. Bone. 2001; 29: 477-86.
24. Battaglino R, Vokes M, Schulze-Späte U, Sharma A, Graves D, Kohler T et al. Fluoxetine treatment increases trabecular bone formation in mice (Fluoxetine affects bone mass). J Cell Biochem. 2007; 100: 1387-94.
25. Bonnet N, Bernard P, Beaupied H, Bizot JC, Trovero F, Courteix D et al. Various effects of antidepressant drugs on bone microarchitectecture, mechanical properties and bone remodeling. Toxicol Appl Pharmacol. 2007; 221: 111-8.
26. Lauder JM, Wilkie MB, Wu C, Singh S. Expression of 5-HT2A, 5-HT2B and 5-HT2C receptors in the mouse embryo. Int J Dev Neurosci. 2000; 8: 653-62.
27. Cavalcanti UDNT, Baratella-Evêncio L, Neto JE, Castro RM, Cardona AS, Melo MLM et al. Morphological aspects of the embryonic development of the TMJ in rats (Rattus norvegicus albinus) treated with fluoxetine. Int J Morphol. 2009; 27: 899-903.
28. Silva IHM, Leão JC, Evêncio LB, Porter SR, De Castro RM. Morphological analysis of the enamel organ in rats treated with fluoxetine. Clinics. 2010; 65: 61-6.
This work is licensed under a Creative Commons Attribution 4.0 International License.
- All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. The online journal is free and open access.